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pgex6p1 ubl sr1 sirpt1  (Addgene inc)


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    Structured Review

    Addgene inc pgex6p1 ubl sr1 sirpt1
    Pgex6p1 Ubl Sr1 Sirpt1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgex6p1+ubl+sr1+sirpt1/pGEX6P1-Ubl-sr1(SIRPT1)+(Plasmid+%2375298)/bio_rxiv__64898__2025__12__18__694329-113-37-44
    Average 93 stars, based on 1 article reviews
    pgex6p1 ubl sr1 sirpt1 - by Bioz Stars, 2026-10
    93/100 stars

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    Plasmid Preparation:

    Article Title: The RNA-binding activity of SACSIN HEPN domain is connected to ARSACS
    Article Snippet: .. The following plasmids were purchased from Addgene: pDEST-cDNA5-FRT/TO-3*Flag-APEX2 N-term was a gift from Benjamin Blencowe (Addgene plasmid # 182925; http://n2t.net/addgene:182925 ; RRID: Addgene_182925); pGEX4T-HEPN was a gift from Kalle Gehring (Addgene plasmid # 75296; http://n2t.net/addgene:75296 ; RRID:Addgene_75296); pGEX6P1-Ubl-sr1(SIRPT1) was a gift from Kalle Gehring (Addgene plasmid # 75298; http://n2t.net/addgene:75298 ; RRID:Addgene_75298). pRetroX GFP Mps1 wt was a gift from Floris Foijer (Addgene plasmid # 63702; http://n2t.net/addgene:63702 ; RRID: Addgene_63702) The cDNA of the HEPN domain was PCR-amplified using the following primers: Forward 5’-GGGGACAAGTTTGTACAAAAAAGCAGGCTCCGTTGGCAATCCAGTGGAAGC-3’; Reverse 5’-GGGGACCACTTTGTACAAGAAAGCTGGGTTTACACTTTTTGTTGCATAAAATTTT CAAGT- 3’. .. The amplified cDNA was cloned into the pDonor201 through BP reaction of the gateway cloning strategy (11789020, ThermoFisher Scientific).

    Polymerase Chain Reaction:

    Article Title: The RNA-binding activity of SACSIN HEPN domain is connected to ARSACS
    Article Snippet: .. The following plasmids were purchased from Addgene: pDEST-cDNA5-FRT/TO-3*Flag-APEX2 N-term was a gift from Benjamin Blencowe (Addgene plasmid # 182925; http://n2t.net/addgene:182925 ; RRID: Addgene_182925); pGEX4T-HEPN was a gift from Kalle Gehring (Addgene plasmid # 75296; http://n2t.net/addgene:75296 ; RRID:Addgene_75296); pGEX6P1-Ubl-sr1(SIRPT1) was a gift from Kalle Gehring (Addgene plasmid # 75298; http://n2t.net/addgene:75298 ; RRID:Addgene_75298). pRetroX GFP Mps1 wt was a gift from Floris Foijer (Addgene plasmid # 63702; http://n2t.net/addgene:63702 ; RRID: Addgene_63702) The cDNA of the HEPN domain was PCR-amplified using the following primers: Forward 5’-GGGGACAAGTTTGTACAAAAAAGCAGGCTCCGTTGGCAATCCAGTGGAAGC-3’; Reverse 5’-GGGGACCACTTTGTACAAGAAAGCTGGGTTTACACTTTTTGTTGCATAAAATTTT CAAGT- 3’. .. The amplified cDNA was cloned into the pDonor201 through BP reaction of the gateway cloning strategy (11789020, ThermoFisher Scientific).



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